Reporter
PlsiR.GFP

Part:BBa_K1725401:Design

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)


PlsiR + B0034 + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 456
    Illegal BsaI.rc site found at 1204


Design Notes

The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction.


Source

The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.

References